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Script Ohio is the signature formation of the Ohio State Marching Band performed before or during halftime, or after home games. According to The Ohio State University Library, a similar floating formation was first performed during the 1932 season by the University of Michigan Marching band, however, it was a set-piece, instead of being formed through marching described below. The Script Ohio is the most identifiable trademark associated with Ohio State Football and the Ohio State Marching Band.

The script is an integrated series of evolutions and formations. The band first forms a triple Block O formation, then slowly unwinds to form the famous letters while playing Robert Planquette’s Le régiment de Sambre et Meuse. The drum major leads the outside O into a peel-off movement around the curves of the script, every musician in continual motion. Slowly the three blocks unfold into a long singular line which loops around, creating the OSUMB’s trademark.

Each time the formation drill is performed, a different fourth or fifth-year sousaphone player has the privilege of standing as the dot in the “i” of “Ohio.” The first sousaphone player to have the honor of dotting the "i" was a fourth year student from Deleware, OH, William Coulter. The dotting of the “i” was ranked the greatest college football tradition by Athlon Sports.

Athlon Sports' 10 Greatest Football Traditions
1. Ohio State's "Dotting of the i"
2. Texas A&M's Bonfire
3. Army-Navy Game
4. Notre Dame's Victory March
5. Michigan's Helmets (stolen from Princeton)
6. Texas-OU Weekend
7. Notre Dame-USC Game
8. The Stanford-Cal Axe
9. The Wishbone
10. The Tournament of Roses

The dotting of the “i” is so important that when the “i”s are dotted during the Double Script Ohio at Michigan Stadium, police protection is needed on the field as Michigan fans have tried to dot the “i” in the past.

Honorary “i”-dotters

Woody Hayes, Bob Hope, Jack Nicklaus, James "Buster" Douglas, OSU Presidents Novice Fawcett, Gordon Gee and their wives, composer Richard "Dick" Heine, retired director Dr. Paul Droste and his wife Ann, and retired OSU ticket director Robert Ries are the select few non-band members who have had the honor of dotting the “i”. This is considered the greatest honor the band can bestow to any non-band member and is an extremely special (and rare) event.